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two-well lab-tek chamber slides  (Thermo Fisher)


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    Thermo Fisher two-well lab-tek chamber slides
    Two Well Lab Tek Chamber Slides, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two-well+lab-tek+chamber+slides/8+well+chamber+slides/pm35353576-389-7-11
    Average 90 stars, based on 1 article reviews
    two-well lab-tek chamber slides - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Single molecule analysis of lamin dynamics
    Article Snippet: To prepare cells for SMT, U2OS cells were seeded into two-well Lab-Tek chamber slides (Thermofisher Scientific) for at least 16 h. Cells were then transiently transfected overnight using 2 μL Lipofectamine 2000 and 0.1 μg of DNA per chamber in OptiMem (Thermofisher Scientific).

    Article Title: Single molecule analysis of lamin dynamics
    Article Snippet: To prepare cells for SMT, U2OS cells were seeded into two-well Lab-Tek chamber slides (Thermofisher Scientific) for at least 16 h. Cells were then transiently transfected overnight using 2 μL Lipofectamine 2000 and 0.5 μg of DNA per chamber in OptiMem (Thermofisher Scientific).

    Article Title: Structural Modeling of GR Interactions with the SWI/SNF Chromatin Remodeling Complex and C/EBP
    Article Snippet: Before glucocorticoid treatments, cells were seeded to two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA) and incubated for at least 18 h in Dulbecco’s modified Eagle’s medium containing 10% charcoal-stripped fetal bovine serum (Hyclone) and 1% L-glutamine.

    Article Title: DNA binding triggers tetramerization of the glucocorticoid receptor in live cells
    Article Snippet: Before glucocorticoid treatments, cells were seeded into two-well Lab-Tek chamber slides (Thermo Fisher) and incubated for at least 18 h in DMEM containing 10% (vol/vol) charcoal-stripped FBS (Life Technologies) and 2 mM l -glutamine.

    Article Title: Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility
    Article Snippet: Single molecule tracking (SMT) Experiments The D4B2D10 cells (or 33AP1C9 cells, expressing HALO-tagged GR) were platted into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA) in Phenol Red free DMEM medium containing 10% charcoal stripped fetal bovine serum (Hyclone, Logan, UT) for at least 18 h. The next day cells were incubated with 0.25nM of cell permeable Janelia Fluor 549 HALOTag ligand [JF549, ( Grimm et al., 2016 )] for 20min.

    Article Title: Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility
    Article Snippet: The D4B2D10 cells (or 33AP1C9 cells, expressing HALO-tagged GR) were platted into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA) in Phenol Red free DMEM medium containing 10% charcoal stripped fetal bovine serum (Hyclone, Logan, UT) for at least 18 h. The next day cells were incubated with 0.25nM of cell permeable Janelia Fluor 549 HALOTag ligand [JF549, ( Grimm et al., 2016 )] for 20min.

    Article Title: The glucocorticoid receptor associates with the cohesin loader NIPBL to promote long-range gene regulation.
    Article Snippet: Briefly, HCT116 RAD21mAID cells were plated into two-well Lab-Tek chamber slides (Thermo Fisher Scientific, Waltham, MA, USA) in Phenol Red–free DMEM medium containing 10% CSS (Hyclone, Logan, UT) and transfected with a Halo-GR plasmid.

    Article Title: Single-molecule analysis of steroid receptor and cofactor action in living cells
    Article Snippet: To stably express their integrated GFP-GR, 3617 cells were grown without tetracycline for 24 h. Prior to hormone treatments, cells were seeded into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA), transiently transfected and incubated for at least 18 h in DMEM medium containing 10% charcoal-stripped FBS (Life Technologies) and 2 mM L -glutamine.

    Plasmid Preparation:

    Article Title: Single molecule analysis of lamin dynamics
    Article Snippet: To prepare cells for SMT, U2OS cells were seeded into two-well Lab-Tek chamber slides (Thermofisher Scientific) for at least 16 h. Cells were then transiently transfected overnight using 2 μL Lipofectamine 2000 and 0.1 μg of DNA per chamber in OptiMem (Thermofisher Scientific).

    Article Title: Single molecule analysis of lamin dynamics
    Article Snippet: To prepare cells for SMT, U2OS cells were seeded into two-well Lab-Tek chamber slides (Thermofisher Scientific) for at least 16 h. Cells were then transiently transfected overnight using 2 μL Lipofectamine 2000 and 0.5 μg of DNA per chamber in OptiMem (Thermofisher Scientific).

    Article Title: Structural Modeling of GR Interactions with the SWI/SNF Chromatin Remodeling Complex and C/EBP
    Article Snippet: Before glucocorticoid treatments, cells were seeded to two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA) and incubated for at least 18 h in Dulbecco’s modified Eagle’s medium containing 10% charcoal-stripped fetal bovine serum (Hyclone) and 1% L-glutamine.

    Article Title: DNA binding triggers tetramerization of the glucocorticoid receptor in live cells
    Article Snippet: Before glucocorticoid treatments, cells were seeded into two-well Lab-Tek chamber slides (Thermo Fisher) and incubated for at least 18 h in DMEM containing 10% (vol/vol) charcoal-stripped FBS (Life Technologies) and 2 mM l -glutamine.

    Article Title: Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility
    Article Snippet: Single molecule tracking (SMT) Experiments The D4B2D10 cells (or 33AP1C9 cells, expressing HALO-tagged GR) were platted into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA) in Phenol Red free DMEM medium containing 10% charcoal stripped fetal bovine serum (Hyclone, Logan, UT) for at least 18 h. The next day cells were incubated with 0.25nM of cell permeable Janelia Fluor 549 HALOTag ligand [JF549, ( Grimm et al., 2016 )] for 20min.

    Article Title: Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility
    Article Snippet: The D4B2D10 cells (or 33AP1C9 cells, expressing HALO-tagged GR) were platted into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA) in Phenol Red free DMEM medium containing 10% charcoal stripped fetal bovine serum (Hyclone, Logan, UT) for at least 18 h. The next day cells were incubated with 0.25nM of cell permeable Janelia Fluor 549 HALOTag ligand [JF549, ( Grimm et al., 2016 )] for 20min.

    Article Title: The glucocorticoid receptor associates with the cohesin loader NIPBL to promote long-range gene regulation.
    Article Snippet: Briefly, HCT116 RAD21mAID cells were plated into two-well Lab-Tek chamber slides (Thermo Fisher Scientific, Waltham, MA, USA) in Phenol Red–free DMEM medium containing 10% CSS (Hyclone, Logan, UT) and transfected with a Halo-GR plasmid.

    Article Title: Single-molecule analysis of steroid receptor and cofactor action in living cells
    Article Snippet: To stably express their integrated GFP-GR, 3617 cells were grown without tetracycline for 24 h. Prior to hormone treatments, cells were seeded into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA), transiently transfected and incubated for at least 18 h in DMEM medium containing 10% charcoal-stripped FBS (Life Technologies) and 2 mM L -glutamine.

    Expressing:

    Article Title: Single molecule analysis of lamin dynamics
    Article Snippet: To prepare cells for SMT, U2OS cells were seeded into two-well Lab-Tek chamber slides (Thermofisher Scientific) for at least 16 h. Cells were then transiently transfected overnight using 2 μL Lipofectamine 2000 and 0.1 μg of DNA per chamber in OptiMem (Thermofisher Scientific).

    Article Title: Single molecule analysis of lamin dynamics
    Article Snippet: To prepare cells for SMT, U2OS cells were seeded into two-well Lab-Tek chamber slides (Thermofisher Scientific) for at least 16 h. Cells were then transiently transfected overnight using 2 μL Lipofectamine 2000 and 0.5 μg of DNA per chamber in OptiMem (Thermofisher Scientific).

    Article Title: Structural Modeling of GR Interactions with the SWI/SNF Chromatin Remodeling Complex and C/EBP
    Article Snippet: Before glucocorticoid treatments, cells were seeded to two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA) and incubated for at least 18 h in Dulbecco’s modified Eagle’s medium containing 10% charcoal-stripped fetal bovine serum (Hyclone) and 1% L-glutamine.

    Article Title: DNA binding triggers tetramerization of the glucocorticoid receptor in live cells
    Article Snippet: Before glucocorticoid treatments, cells were seeded into two-well Lab-Tek chamber slides (Thermo Fisher) and incubated for at least 18 h in DMEM containing 10% (vol/vol) charcoal-stripped FBS (Life Technologies) and 2 mM l -glutamine.

    Article Title: Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility
    Article Snippet: Single molecule tracking (SMT) Experiments The D4B2D10 cells (or 33AP1C9 cells, expressing HALO-tagged GR) were platted into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA) in Phenol Red free DMEM medium containing 10% charcoal stripped fetal bovine serum (Hyclone, Logan, UT) for at least 18 h. The next day cells were incubated with 0.25nM of cell permeable Janelia Fluor 549 HALOTag ligand [JF549, ( Grimm et al., 2016 )] for 20min.

    Article Title: Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility
    Article Snippet: The D4B2D10 cells (or 33AP1C9 cells, expressing HALO-tagged GR) were platted into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA) in Phenol Red free DMEM medium containing 10% charcoal stripped fetal bovine serum (Hyclone, Logan, UT) for at least 18 h. The next day cells were incubated with 0.25nM of cell permeable Janelia Fluor 549 HALOTag ligand [JF549, ( Grimm et al., 2016 )] for 20min.

    Article Title: The glucocorticoid receptor associates with the cohesin loader NIPBL to promote long-range gene regulation.
    Article Snippet: Briefly, HCT116 RAD21mAID cells were plated into two-well Lab-Tek chamber slides (Thermo Fisher Scientific, Waltham, MA, USA) in Phenol Red–free DMEM medium containing 10% CSS (Hyclone, Logan, UT) and transfected with a Halo-GR plasmid.

    Article Title: Single-molecule analysis of steroid receptor and cofactor action in living cells
    Article Snippet: To stably express their integrated GFP-GR, 3617 cells were grown without tetracycline for 24 h. Prior to hormone treatments, cells were seeded into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA), transiently transfected and incubated for at least 18 h in DMEM medium containing 10% charcoal-stripped FBS (Life Technologies) and 2 mM L -glutamine.

    Incubation:

    Article Title: Single molecule analysis of lamin dynamics
    Article Snippet: To prepare cells for SMT, U2OS cells were seeded into two-well Lab-Tek chamber slides (Thermofisher Scientific) for at least 16 h. Cells were then transiently transfected overnight using 2 μL Lipofectamine 2000 and 0.1 μg of DNA per chamber in OptiMem (Thermofisher Scientific).

    Article Title: Single molecule analysis of lamin dynamics
    Article Snippet: To prepare cells for SMT, U2OS cells were seeded into two-well Lab-Tek chamber slides (Thermofisher Scientific) for at least 16 h. Cells were then transiently transfected overnight using 2 μL Lipofectamine 2000 and 0.5 μg of DNA per chamber in OptiMem (Thermofisher Scientific).

    Article Title: Structural Modeling of GR Interactions with the SWI/SNF Chromatin Remodeling Complex and C/EBP
    Article Snippet: Before glucocorticoid treatments, cells were seeded to two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA) and incubated for at least 18 h in Dulbecco’s modified Eagle’s medium containing 10% charcoal-stripped fetal bovine serum (Hyclone) and 1% L-glutamine.

    Article Title: DNA binding triggers tetramerization of the glucocorticoid receptor in live cells
    Article Snippet: Before glucocorticoid treatments, cells were seeded into two-well Lab-Tek chamber slides (Thermo Fisher) and incubated for at least 18 h in DMEM containing 10% (vol/vol) charcoal-stripped FBS (Life Technologies) and 2 mM l -glutamine.

    Article Title: Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility
    Article Snippet: Single molecule tracking (SMT) Experiments The D4B2D10 cells (or 33AP1C9 cells, expressing HALO-tagged GR) were platted into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA) in Phenol Red free DMEM medium containing 10% charcoal stripped fetal bovine serum (Hyclone, Logan, UT) for at least 18 h. The next day cells were incubated with 0.25nM of cell permeable Janelia Fluor 549 HALOTag ligand [JF549, ( Grimm et al., 2016 )] for 20min.

    Article Title: Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility
    Article Snippet: The D4B2D10 cells (or 33AP1C9 cells, expressing HALO-tagged GR) were platted into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA) in Phenol Red free DMEM medium containing 10% charcoal stripped fetal bovine serum (Hyclone, Logan, UT) for at least 18 h. The next day cells were incubated with 0.25nM of cell permeable Janelia Fluor 549 HALOTag ligand [JF549, ( Grimm et al., 2016 )] for 20min.

    Article Title: The glucocorticoid receptor associates with the cohesin loader NIPBL to promote long-range gene regulation.
    Article Snippet: Briefly, HCT116 RAD21mAID cells were plated into two-well Lab-Tek chamber slides (Thermo Fisher Scientific, Waltham, MA, USA) in Phenol Red–free DMEM medium containing 10% CSS (Hyclone, Logan, UT) and transfected with a Halo-GR plasmid.

    Article Title: Single-molecule analysis of steroid receptor and cofactor action in living cells
    Article Snippet: To stably express their integrated GFP-GR, 3617 cells were grown without tetracycline for 24 h. Prior to hormone treatments, cells were seeded into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA), transiently transfected and incubated for at least 18 h in DMEM medium containing 10% charcoal-stripped FBS (Life Technologies) and 2 mM L -glutamine.

    Stable Transfection:

    Article Title: Single molecule analysis of lamin dynamics
    Article Snippet: To prepare cells for SMT, U2OS cells were seeded into two-well Lab-Tek chamber slides (Thermofisher Scientific) for at least 16 h. Cells were then transiently transfected overnight using 2 μL Lipofectamine 2000 and 0.1 μg of DNA per chamber in OptiMem (Thermofisher Scientific).

    Article Title: Single molecule analysis of lamin dynamics
    Article Snippet: To prepare cells for SMT, U2OS cells were seeded into two-well Lab-Tek chamber slides (Thermofisher Scientific) for at least 16 h. Cells were then transiently transfected overnight using 2 μL Lipofectamine 2000 and 0.5 μg of DNA per chamber in OptiMem (Thermofisher Scientific).

    Article Title: Structural Modeling of GR Interactions with the SWI/SNF Chromatin Remodeling Complex and C/EBP
    Article Snippet: Before glucocorticoid treatments, cells were seeded to two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA) and incubated for at least 18 h in Dulbecco’s modified Eagle’s medium containing 10% charcoal-stripped fetal bovine serum (Hyclone) and 1% L-glutamine.

    Article Title: DNA binding triggers tetramerization of the glucocorticoid receptor in live cells
    Article Snippet: Before glucocorticoid treatments, cells were seeded into two-well Lab-Tek chamber slides (Thermo Fisher) and incubated for at least 18 h in DMEM containing 10% (vol/vol) charcoal-stripped FBS (Life Technologies) and 2 mM l -glutamine.

    Article Title: Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility
    Article Snippet: Single molecule tracking (SMT) Experiments The D4B2D10 cells (or 33AP1C9 cells, expressing HALO-tagged GR) were platted into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA) in Phenol Red free DMEM medium containing 10% charcoal stripped fetal bovine serum (Hyclone, Logan, UT) for at least 18 h. The next day cells were incubated with 0.25nM of cell permeable Janelia Fluor 549 HALOTag ligand [JF549, ( Grimm et al., 2016 )] for 20min.

    Article Title: Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility
    Article Snippet: The D4B2D10 cells (or 33AP1C9 cells, expressing HALO-tagged GR) were platted into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA) in Phenol Red free DMEM medium containing 10% charcoal stripped fetal bovine serum (Hyclone, Logan, UT) for at least 18 h. The next day cells were incubated with 0.25nM of cell permeable Janelia Fluor 549 HALOTag ligand [JF549, ( Grimm et al., 2016 )] for 20min.

    Article Title: The glucocorticoid receptor associates with the cohesin loader NIPBL to promote long-range gene regulation.
    Article Snippet: Briefly, HCT116 RAD21mAID cells were plated into two-well Lab-Tek chamber slides (Thermo Fisher Scientific, Waltham, MA, USA) in Phenol Red–free DMEM medium containing 10% CSS (Hyclone, Logan, UT) and transfected with a Halo-GR plasmid.

    Article Title: Single-molecule analysis of steroid receptor and cofactor action in living cells
    Article Snippet: To stably express their integrated GFP-GR, 3617 cells were grown without tetracycline for 24 h. Prior to hormone treatments, cells were seeded into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA), transiently transfected and incubated for at least 18 h in DMEM medium containing 10% charcoal-stripped FBS (Life Technologies) and 2 mM L -glutamine.

    Modification:

    Article Title: Single molecule analysis of lamin dynamics
    Article Snippet: To prepare cells for SMT, U2OS cells were seeded into two-well Lab-Tek chamber slides (Thermofisher Scientific) for at least 16 h. Cells were then transiently transfected overnight using 2 μL Lipofectamine 2000 and 0.1 μg of DNA per chamber in OptiMem (Thermofisher Scientific).

    Article Title: Single molecule analysis of lamin dynamics
    Article Snippet: To prepare cells for SMT, U2OS cells were seeded into two-well Lab-Tek chamber slides (Thermofisher Scientific) for at least 16 h. Cells were then transiently transfected overnight using 2 μL Lipofectamine 2000 and 0.5 μg of DNA per chamber in OptiMem (Thermofisher Scientific).

    Article Title: Structural Modeling of GR Interactions with the SWI/SNF Chromatin Remodeling Complex and C/EBP
    Article Snippet: Before glucocorticoid treatments, cells were seeded to two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA) and incubated for at least 18 h in Dulbecco’s modified Eagle’s medium containing 10% charcoal-stripped fetal bovine serum (Hyclone) and 1% L-glutamine.

    Article Title: DNA binding triggers tetramerization of the glucocorticoid receptor in live cells
    Article Snippet: Before glucocorticoid treatments, cells were seeded into two-well Lab-Tek chamber slides (Thermo Fisher) and incubated for at least 18 h in DMEM containing 10% (vol/vol) charcoal-stripped FBS (Life Technologies) and 2 mM l -glutamine.

    Article Title: Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility
    Article Snippet: Single molecule tracking (SMT) Experiments The D4B2D10 cells (or 33AP1C9 cells, expressing HALO-tagged GR) were platted into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA) in Phenol Red free DMEM medium containing 10% charcoal stripped fetal bovine serum (Hyclone, Logan, UT) for at least 18 h. The next day cells were incubated with 0.25nM of cell permeable Janelia Fluor 549 HALOTag ligand [JF549, ( Grimm et al., 2016 )] for 20min.

    Article Title: Transcriptional Bursting and Co-bursting Regulation by Steroid Hormone Release Pattern and Transcription Factor Mobility
    Article Snippet: The D4B2D10 cells (or 33AP1C9 cells, expressing HALO-tagged GR) were platted into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA) in Phenol Red free DMEM medium containing 10% charcoal stripped fetal bovine serum (Hyclone, Logan, UT) for at least 18 h. The next day cells were incubated with 0.25nM of cell permeable Janelia Fluor 549 HALOTag ligand [JF549, ( Grimm et al., 2016 )] for 20min.

    Article Title: The glucocorticoid receptor associates with the cohesin loader NIPBL to promote long-range gene regulation.
    Article Snippet: Briefly, HCT116 RAD21mAID cells were plated into two-well Lab-Tek chamber slides (Thermo Fisher Scientific, Waltham, MA, USA) in Phenol Red–free DMEM medium containing 10% CSS (Hyclone, Logan, UT) and transfected with a Halo-GR plasmid.

    Article Title: Single-molecule analysis of steroid receptor and cofactor action in living cells
    Article Snippet: To stably express their integrated GFP-GR, 3617 cells were grown without tetracycline for 24 h. Prior to hormone treatments, cells were seeded into two-well Lab-Tek chamber slides (Thermo Fisher, Waltham, MA, USA), transiently transfected and incubated for at least 18 h in DMEM medium containing 10% charcoal-stripped FBS (Life Technologies) and 2 mM L -glutamine.



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